Effects of Extraction Solvents on Phytochemical Screening, Cytotoxicity and Anti-Obesity Activities of Selected Sri Lankan Medicinal Plants

Pharmacognosy Research,2021,13,4,246-256.DOI:10.5530/pres.13.4.18Published:October 2021Type:Original ArticleAuthors:Silva Waruni Randika, Nugara Ruwani Nilushi, Manage Pathmalal, Undugoda Lanka, Udayanga Dhanushka, Liyanage Renuka Nilmini, and Galhena Prasanna Author(s) affiliations:Silva Randika1,2, Nilushi1,*, Pathmalal2,3, Lanka1, Dhanushka1, Nilmini4, Prasanna5 1Department of Biosystems Technology, Faculty University Sri Jayewardenepura, Gangodawila, Nugegoda, SRI LANKA. 2Faculty Graduate Studies, 3Centre for Water Quality Algae Research, Department Zoology, Applied Sciences, 4Department Materials Mechanical 5Department Biochemistry Clinical Chemistry, Medicine, Kelaniya, Abstract:Background: Phytoconstituents are known to have an arena health benefits. The choice extraction solvents a desired pharmacological activity is poorly characterized. Objectives: present study compared the use different extract phytoconstituents, evaluate cytotoxicity anti-obesity activities. Methods: Leaves, bark, flowers or tubers fourteen commonly utilized plants in Lankan Ayurvedic system were used prepare extracts distilled water (DW), ethanol n-hexane. Phytochemical screening, total phenolic flavonoid contents (TPC TFC), triglyceride assays performed. Results: DW served as best solvent highest yield phytochemicals followed by marking hexane least efficient solvent. (WE) Averrhoa bilimbi leaves (EE) Cassia auriculata showed TPC. TFC was lowest (HE) all plants. WE ≥75% cell viability below 500 μg/mL with no potential anti-adipogenic activity. EE Justicia adhathoda maximum lipid inhibition (88.31%), Aerva lanata (74.65%), Cyperus rotundus (74.04%) at 100 μg/mL. Ipomoea aquatica (45.11%), Murrya koenigii (41.04%), Hemidesmus indicus (32.97%) viable anti-adiposity 50 Conclusion: most provided greater phytoconstituents enabling their safe usage higher doses during medication. Ethanol better constituents responsible anti-adipogenesis possible correlation found between content anti-adiposity. Keywords:3T3-L1, Anti-adipogenesis, Ayurveda, cytotoxicity, Medicinal plants, PhytochemicalsView:PDF (2.15 MB)